human hepatocyte cells (hh) Search Results


methylcellulose plates methocult gf h4535  (CellSystems Biotechnologie Vertrieb GmbH)
90
CellSystems Biotechnologie Vertrieb GmbH methylcellulose plates methocult gf h4535
Methylcellulose Plates Methocult Gf H4535, supplied by CellSystems Biotechnologie Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+hepatocyte+cells+%28hh%29/us08309608-52-27-45?v=CellSystems+Biotechnologie+Vertrieb+GmbH
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methylcellulose plates methocult gf h4535 - by Bioz Stars, 2026-07
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human hepatocyte cells (hh)  (ScienCell)
90
ScienCell human hepatocyte cells (hh)
Effects of AuNPs + radiation combined treatment on the cell proliferation of normal hepatocytes and hepatic stellate cell line. (A) MTT assays were performed using HH cells treated with AuNPs or X-ray irradiation. (B) Transmission electron microscopy images of AuNPs uptake inside the cell. (C) MTT assays were performed using <t>hepatocyte</t> cells (HH: left panel) and hepatic stellate cell line (LX-2: right panel) treated with AuNPs or X-ray irradiation, or a combination of the two treatments. The blue arrows indicate the magnification of AuNPs. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; HH, human hepatocyte cell.
Human Hepatocyte Cells (Hh), supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+hepatocyte+cells+%28hh%29/pmc09459506-26-0-7?v=ScienCell
Average 90 stars, based on 1 article reviews
human hepatocyte cells (hh) - by Bioz Stars, 2026-07
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human primary hepatocytes (hph)  (ScienCell)
90
ScienCell human primary hepatocytes (hph)
Effects of AuNPs + radiation combined treatment on the cell proliferation of normal hepatocytes and hepatic stellate cell line. (A) MTT assays were performed using HH cells treated with AuNPs or X-ray irradiation. (B) Transmission electron microscopy images of AuNPs uptake inside the cell. (C) MTT assays were performed using <t>hepatocyte</t> cells (HH: left panel) and hepatic stellate cell line (LX-2: right panel) treated with AuNPs or X-ray irradiation, or a combination of the two treatments. The blue arrows indicate the magnification of AuNPs. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; HH, human hepatocyte cell.
Human Primary Hepatocytes (Hph), supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+hepatocyte+cells+%28hh%29/10__1158_slash_1535___7163__mct___15___0580-73-0-7?v=ScienCell
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human primary hepatocytes (hph) - by Bioz Stars, 2026-07
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cryopreserved phh  (PRIMACYT Cell Culture Technology GmbH)
90
PRIMACYT Cell Culture Technology GmbH cryopreserved phh
Effects of AuNPs + radiation combined treatment on the cell proliferation of normal hepatocytes and hepatic stellate cell line. (A) MTT assays were performed using HH cells treated with AuNPs or X-ray irradiation. (B) Transmission electron microscopy images of AuNPs uptake inside the cell. (C) MTT assays were performed using <t>hepatocyte</t> cells (HH: left panel) and hepatic stellate cell line (LX-2: right panel) treated with AuNPs or X-ray irradiation, or a combination of the two treatments. The blue arrows indicate the magnification of AuNPs. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; HH, human hepatocyte cell.
Cryopreserved Phh, supplied by PRIMACYT Cell Culture Technology GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+hepatocyte+cells+%28hh%29/pmc07179450-213-0-6?v=PRIMACYT+Cell+Culture+Technology+GmbH
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cryopreserved phh - by Bioz Stars, 2026-07
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primary human hepatocyte  (PhoenixBio Co)
90
PhoenixBio Co primary human hepatocyte
(A) Relative mRNA expression of VLDLR in various tissues was determined using the NextBio Body Atlas application. (B) mRNA expression of SR-B1, LDLR, VLDLR and GAPDH in Huh7 cells and primary human <t>hepatocyte</t> <t>(PHH)</t> were determined by qRT-PCR. Relative expression levels of mRNA were calculated based on the expression level of GAPDH. (C) VLDLR-HA was exogenously expressed in parental, SR-KO, LD-KO and SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR in these cells were determined by immunoblotting analysis (upper panel). Parental, CD81 KO and SR/LD-DKO Huh7 cells expressing SR-B1, LDLR or VLDLR were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection (lower panel). (D) VLDLR-HA was exogenously expressed in CD81 KO, CLDN1 KO and OCLN KO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR, CD81, CLDN1 and OCLN in these cells were determined by immunoblotting analysis (upper panel). Cells were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR (lower panel). (E) SR-B1, LDLR and VLDLR were exogenously expressed in SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Cells were infected with Con1-JFH1 or Jc1 at an MOI of 1, and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR. (F) Sera (100μl) from chimeric mice infected with HCV were inoculated into SR/LD-DKO Huh7 cells expressing either SR-B1, LDLR or VLDLR in 24 well plate. Intracellular HCV RNA levels were determined at 72 h post-infection. In all cases, asterisks indicate significant differences (*P<0.05; **P<0.01) versus the results for control cells.
Primary Human Hepatocyte, supplied by PhoenixBio Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+hepatocyte+cells+%28hh%29/pmc04859476-192-2-8?v=PhoenixBio+Co
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primary human hepatocyte - by Bioz Stars, 2026-07
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primary human hepatocytes  (PRIMACYT Cell Culture Technology GmbH)
90
PRIMACYT Cell Culture Technology GmbH primary human hepatocytes
(A) Relative mRNA expression of VLDLR in various tissues was determined using the NextBio Body Atlas application. (B) mRNA expression of SR-B1, LDLR, VLDLR and GAPDH in Huh7 cells and primary human <t>hepatocyte</t> <t>(PHH)</t> were determined by qRT-PCR. Relative expression levels of mRNA were calculated based on the expression level of GAPDH. (C) VLDLR-HA was exogenously expressed in parental, SR-KO, LD-KO and SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR in these cells were determined by immunoblotting analysis (upper panel). Parental, CD81 KO and SR/LD-DKO Huh7 cells expressing SR-B1, LDLR or VLDLR were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection (lower panel). (D) VLDLR-HA was exogenously expressed in CD81 KO, CLDN1 KO and OCLN KO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR, CD81, CLDN1 and OCLN in these cells were determined by immunoblotting analysis (upper panel). Cells were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR (lower panel). (E) SR-B1, LDLR and VLDLR were exogenously expressed in SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Cells were infected with Con1-JFH1 or Jc1 at an MOI of 1, and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR. (F) Sera (100μl) from chimeric mice infected with HCV were inoculated into SR/LD-DKO Huh7 cells expressing either SR-B1, LDLR or VLDLR in 24 well plate. Intracellular HCV RNA levels were determined at 72 h post-infection. In all cases, asterisks indicate significant differences (*P<0.05; **P<0.01) versus the results for control cells.
Primary Human Hepatocytes, supplied by PRIMACYT Cell Culture Technology GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+hepatocyte+cells+%28hh%29/us12024708-220-0-3?v=PRIMACYT+Cell+Culture+Technology+GmbH
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primary human hepatocytes - by Bioz Stars, 2026-07
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il 6 d6050  (R&D Systems)
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R&D Systems il 6 d6050
(A) Relative mRNA expression of VLDLR in various tissues was determined using the NextBio Body Atlas application. (B) mRNA expression of SR-B1, LDLR, VLDLR and GAPDH in Huh7 cells and primary human <t>hepatocyte</t> <t>(PHH)</t> were determined by qRT-PCR. Relative expression levels of mRNA were calculated based on the expression level of GAPDH. (C) VLDLR-HA was exogenously expressed in parental, SR-KO, LD-KO and SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR in these cells were determined by immunoblotting analysis (upper panel). Parental, CD81 KO and SR/LD-DKO Huh7 cells expressing SR-B1, LDLR or VLDLR were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection (lower panel). (D) VLDLR-HA was exogenously expressed in CD81 KO, CLDN1 KO and OCLN KO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR, CD81, CLDN1 and OCLN in these cells were determined by immunoblotting analysis (upper panel). Cells were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR (lower panel). (E) SR-B1, LDLR and VLDLR were exogenously expressed in SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Cells were infected with Con1-JFH1 or Jc1 at an MOI of 1, and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR. (F) Sera (100μl) from chimeric mice infected with HCV were inoculated into SR/LD-DKO Huh7 cells expressing either SR-B1, LDLR or VLDLR in 24 well plate. Intracellular HCV RNA levels were determined at 72 h post-infection. In all cases, asterisks indicate significant differences (*P<0.05; **P<0.01) versus the results for control cells.
Il 6 D6050, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+hepatocyte+cells+%28hh%29/pmc03628221-151-10-4?v=R%26D+Systems
Average 96 stars, based on 1 article reviews
il 6 d6050 - by Bioz Stars, 2026-07
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cold air 827 il 6  (R&D Systems)
97
R&D Systems cold air 827 il 6
(A) Relative mRNA expression of VLDLR in various tissues was determined using the NextBio Body Atlas application. (B) mRNA expression of SR-B1, LDLR, VLDLR and GAPDH in Huh7 cells and primary human <t>hepatocyte</t> <t>(PHH)</t> were determined by qRT-PCR. Relative expression levels of mRNA were calculated based on the expression level of GAPDH. (C) VLDLR-HA was exogenously expressed in parental, SR-KO, LD-KO and SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR in these cells were determined by immunoblotting analysis (upper panel). Parental, CD81 KO and SR/LD-DKO Huh7 cells expressing SR-B1, LDLR or VLDLR were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection (lower panel). (D) VLDLR-HA was exogenously expressed in CD81 KO, CLDN1 KO and OCLN KO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR, CD81, CLDN1 and OCLN in these cells were determined by immunoblotting analysis (upper panel). Cells were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR (lower panel). (E) SR-B1, LDLR and VLDLR were exogenously expressed in SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Cells were infected with Con1-JFH1 or Jc1 at an MOI of 1, and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR. (F) Sera (100μl) from chimeric mice infected with HCV were inoculated into SR/LD-DKO Huh7 cells expressing either SR-B1, LDLR or VLDLR in 24 well plate. Intracellular HCV RNA levels were determined at 72 h post-infection. In all cases, asterisks indicate significant differences (*P<0.05; **P<0.01) versus the results for control cells.
Cold Air 827 Il 6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+hepatocyte+cells+%28hh%29/pm09817153-91-12-16?v=R%26D+Systems
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cold air 827 il 6 - by Bioz Stars, 2026-07
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v-plex human pro-inflammatory panel 1  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC v-plex human pro-inflammatory panel 1
(A) Relative mRNA expression of VLDLR in various tissues was determined using the NextBio Body Atlas application. (B) mRNA expression of SR-B1, LDLR, VLDLR and GAPDH in Huh7 cells and primary human <t>hepatocyte</t> <t>(PHH)</t> were determined by qRT-PCR. Relative expression levels of mRNA were calculated based on the expression level of GAPDH. (C) VLDLR-HA was exogenously expressed in parental, SR-KO, LD-KO and SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR in these cells were determined by immunoblotting analysis (upper panel). Parental, CD81 KO and SR/LD-DKO Huh7 cells expressing SR-B1, LDLR or VLDLR were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection (lower panel). (D) VLDLR-HA was exogenously expressed in CD81 KO, CLDN1 KO and OCLN KO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR, CD81, CLDN1 and OCLN in these cells were determined by immunoblotting analysis (upper panel). Cells were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR (lower panel). (E) SR-B1, LDLR and VLDLR were exogenously expressed in SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Cells were infected with Con1-JFH1 or Jc1 at an MOI of 1, and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR. (F) Sera (100μl) from chimeric mice infected with HCV were inoculated into SR/LD-DKO Huh7 cells expressing either SR-B1, LDLR or VLDLR in 24 well plate. Intracellular HCV RNA levels were determined at 72 h post-infection. In all cases, asterisks indicate significant differences (*P<0.05; **P<0.01) versus the results for control cells.
V Plex Human Pro Inflammatory Panel 1, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+hepatocyte+cells+%28hh%29/pmc07029330-133-18-11?v=Meso+Scale+Diagnostics+LLC
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v-plex human pro-inflammatory panel 1 - by Bioz Stars, 2026-07
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kreb s ringer buffer primary hepatocytes  (Thermo Fisher)
97
Thermo Fisher kreb s ringer buffer primary hepatocytes
(A) Relative mRNA expression of VLDLR in various tissues was determined using the NextBio Body Atlas application. (B) mRNA expression of SR-B1, LDLR, VLDLR and GAPDH in Huh7 cells and primary human <t>hepatocyte</t> <t>(PHH)</t> were determined by qRT-PCR. Relative expression levels of mRNA were calculated based on the expression level of GAPDH. (C) VLDLR-HA was exogenously expressed in parental, SR-KO, LD-KO and SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR in these cells were determined by immunoblotting analysis (upper panel). Parental, CD81 KO and SR/LD-DKO Huh7 cells expressing SR-B1, LDLR or VLDLR were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection (lower panel). (D) VLDLR-HA was exogenously expressed in CD81 KO, CLDN1 KO and OCLN KO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR, CD81, CLDN1 and OCLN in these cells were determined by immunoblotting analysis (upper panel). Cells were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR (lower panel). (E) SR-B1, LDLR and VLDLR were exogenously expressed in SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Cells were infected with Con1-JFH1 or Jc1 at an MOI of 1, and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR. (F) Sera (100μl) from chimeric mice infected with HCV were inoculated into SR/LD-DKO Huh7 cells expressing either SR-B1, LDLR or VLDLR in 24 well plate. Intracellular HCV RNA levels were determined at 72 h post-infection. In all cases, asterisks indicate significant differences (*P<0.05; **P<0.01) versus the results for control cells.
Kreb S Ringer Buffer Primary Hepatocytes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+hepatocyte+cells+%28hh%29/us09822338-1171-45-70?v=Thermo+Fisher
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kreb s ringer buffer primary hepatocytes - by Bioz Stars, 2026-07
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il 6  (R&D Systems)
94
R&D Systems il 6
(A) Relative mRNA expression of VLDLR in various tissues was determined using the NextBio Body Atlas application. (B) mRNA expression of SR-B1, LDLR, VLDLR and GAPDH in Huh7 cells and primary human <t>hepatocyte</t> <t>(PHH)</t> were determined by qRT-PCR. Relative expression levels of mRNA were calculated based on the expression level of GAPDH. (C) VLDLR-HA was exogenously expressed in parental, SR-KO, LD-KO and SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR in these cells were determined by immunoblotting analysis (upper panel). Parental, CD81 KO and SR/LD-DKO Huh7 cells expressing SR-B1, LDLR or VLDLR were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection (lower panel). (D) VLDLR-HA was exogenously expressed in CD81 KO, CLDN1 KO and OCLN KO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR, CD81, CLDN1 and OCLN in these cells were determined by immunoblotting analysis (upper panel). Cells were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR (lower panel). (E) SR-B1, LDLR and VLDLR were exogenously expressed in SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Cells were infected with Con1-JFH1 or Jc1 at an MOI of 1, and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR. (F) Sera (100μl) from chimeric mice infected with HCV were inoculated into SR/LD-DKO Huh7 cells expressing either SR-B1, LDLR or VLDLR in 24 well plate. Intracellular HCV RNA levels were determined at 72 h post-infection. In all cases, asterisks indicate significant differences (*P<0.05; **P<0.01) versus the results for control cells.
Il 6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+hepatocyte+cells+%28hh%29/10__1074_slash_jbc__m111__257071-72-13-9?v=R%26D+Systems
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il 6 - by Bioz Stars, 2026-07
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anti interleukin il 6  (Bioss)
93
Bioss anti interleukin il 6
Typical expression images of immunochemistry staining in tissue microarrays. (A and B) Low expression of CD68 in MIA tissues. Original magnification, (A) ×100 and (B) ×200. (C and D) High expression of CD68 in LPA tissues. Original magnification, (C) ×100 and (D) ×200. (E) Negative expression <t>of</t> <t>IL-6</t> in MIA tissues. Original magnification, ×200. (F) Positive expression of IL-6 in LPA tissues. Original magnification, ×200. (G) Negative expression of CSF-1 in MIA tissues. Original magnification, ×200. (H) Positive expression of CSF-1 in LPA tissues. Original magnification, ×200. (I) High E-cadherin expression in MIA tissues. Original magnification, ×100. (J) Low E-cadherin expression in LPA tissues. Original magnification, ×100. (K) High Snail expression in LPA tissues. Original magnification, ×100. (L) High MMP-2 expression in LPA tissues. Original magnification, ×100. CD, cluster of differentiation; MIA, minimally invasive adenocarcinoma; LPA, lepidic predominant adenocarcinoma; CSF, colony-stimulating factor; IL, <t>interleukin;</t> MMP, metalloproteinase.
Anti Interleukin Il 6, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Effects of AuNPs + radiation combined treatment on the cell proliferation of normal hepatocytes and hepatic stellate cell line. (A) MTT assays were performed using HH cells treated with AuNPs or X-ray irradiation. (B) Transmission electron microscopy images of AuNPs uptake inside the cell. (C) MTT assays were performed using hepatocyte cells (HH: left panel) and hepatic stellate cell line (LX-2: right panel) treated with AuNPs or X-ray irradiation, or a combination of the two treatments. The blue arrows indicate the magnification of AuNPs. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; HH, human hepatocyte cell.

Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: Effects of AuNPs + radiation combined treatment on the cell proliferation of normal hepatocytes and hepatic stellate cell line. (A) MTT assays were performed using HH cells treated with AuNPs or X-ray irradiation. (B) Transmission electron microscopy images of AuNPs uptake inside the cell. (C) MTT assays were performed using hepatocyte cells (HH: left panel) and hepatic stellate cell line (LX-2: right panel) treated with AuNPs or X-ray irradiation, or a combination of the two treatments. The blue arrows indicate the magnification of AuNPs. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; HH, human hepatocyte cell.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Irradiation, Transmission Assay, Electron Microscopy

Cell SF of AuNPs + radiation combined treatment of normal hepatocytes. Clonogenic assay of hepatocytes after treatment with AuNPs or X-ray irradiation or a combination of the two treatments. SF, survival fraction; IR, irradiation.

Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: Cell SF of AuNPs + radiation combined treatment of normal hepatocytes. Clonogenic assay of hepatocytes after treatment with AuNPs or X-ray irradiation or a combination of the two treatments. SF, survival fraction; IR, irradiation.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Clonogenic Assay, Irradiation

Effects on apoptosis of AuNPs + radiation combined treatment of hepatocytes. (A) Cells were treated with PI at 48 h post-treatment after each indicated treatment and analyzed by FACS. (B) The indicated antibodies were used for western blotting of hepatocytes treated with AuNPs or X-ray irradiation or a combination of the two treatments. Values represent the means of 3 experiments ± SD. (C) Cells were treated with AuNPs and/or X-ray irradiation or a combination of the two treatments for 24 h. The cell-cycle distribution of subG1 was analyzed quantitatively. *, P<0.05 and **, P<0.01. IR, irradiation; PARP, poly (ADP-ribose) polymerase; PI, propidium iodide; FACS, fluorescence-activated cell sorting.

Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: Effects on apoptosis of AuNPs + radiation combined treatment of hepatocytes. (A) Cells were treated with PI at 48 h post-treatment after each indicated treatment and analyzed by FACS. (B) The indicated antibodies were used for western blotting of hepatocytes treated with AuNPs or X-ray irradiation or a combination of the two treatments. Values represent the means of 3 experiments ± SD. (C) Cells were treated with AuNPs and/or X-ray irradiation or a combination of the two treatments for 24 h. The cell-cycle distribution of subG1 was analyzed quantitatively. *, P<0.05 and **, P<0.01. IR, irradiation; PARP, poly (ADP-ribose) polymerase; PI, propidium iodide; FACS, fluorescence-activated cell sorting.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Western Blot, Irradiation, Fluorescence, FACS

IL-8 concentrations secreted from normal hepatocytes after AuNPs + X-ray irradiation combined treatment. In all therapeutic groups, a decrease in the amount of IL-8 was observed in the radiation alone group. The combination therapy did not lead to significantly lower levels of IL-8 than radiation alone. IL-8, interleukin-8; IR, irradiation.

Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: IL-8 concentrations secreted from normal hepatocytes after AuNPs + X-ray irradiation combined treatment. In all therapeutic groups, a decrease in the amount of IL-8 was observed in the radiation alone group. The combination therapy did not lead to significantly lower levels of IL-8 than radiation alone. IL-8, interleukin-8; IR, irradiation.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Irradiation

(A) Relative mRNA expression of VLDLR in various tissues was determined using the NextBio Body Atlas application. (B) mRNA expression of SR-B1, LDLR, VLDLR and GAPDH in Huh7 cells and primary human hepatocyte (PHH) were determined by qRT-PCR. Relative expression levels of mRNA were calculated based on the expression level of GAPDH. (C) VLDLR-HA was exogenously expressed in parental, SR-KO, LD-KO and SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR in these cells were determined by immunoblotting analysis (upper panel). Parental, CD81 KO and SR/LD-DKO Huh7 cells expressing SR-B1, LDLR or VLDLR were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection (lower panel). (D) VLDLR-HA was exogenously expressed in CD81 KO, CLDN1 KO and OCLN KO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR, CD81, CLDN1 and OCLN in these cells were determined by immunoblotting analysis (upper panel). Cells were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR (lower panel). (E) SR-B1, LDLR and VLDLR were exogenously expressed in SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Cells were infected with Con1-JFH1 or Jc1 at an MOI of 1, and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR. (F) Sera (100μl) from chimeric mice infected with HCV were inoculated into SR/LD-DKO Huh7 cells expressing either SR-B1, LDLR or VLDLR in 24 well plate. Intracellular HCV RNA levels were determined at 72 h post-infection. In all cases, asterisks indicate significant differences (*P<0.05; **P<0.01) versus the results for control cells.

Journal: PLoS Pathogens

Article Title: Lipoprotein Receptors Redundantly Participate in Entry of Hepatitis C Virus

doi: 10.1371/journal.ppat.1005610

Figure Lengend Snippet: (A) Relative mRNA expression of VLDLR in various tissues was determined using the NextBio Body Atlas application. (B) mRNA expression of SR-B1, LDLR, VLDLR and GAPDH in Huh7 cells and primary human hepatocyte (PHH) were determined by qRT-PCR. Relative expression levels of mRNA were calculated based on the expression level of GAPDH. (C) VLDLR-HA was exogenously expressed in parental, SR-KO, LD-KO and SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR in these cells were determined by immunoblotting analysis (upper panel). Parental, CD81 KO and SR/LD-DKO Huh7 cells expressing SR-B1, LDLR or VLDLR were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection (lower panel). (D) VLDLR-HA was exogenously expressed in CD81 KO, CLDN1 KO and OCLN KO Huh7 cells by infection with lentiviral vectors. Expressions of VLDLR, CD81, CLDN1 and OCLN in these cells were determined by immunoblotting analysis (upper panel). Cells were infected with HCVcc at an MOI of 1 and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR (lower panel). (E) SR-B1, LDLR and VLDLR were exogenously expressed in SR/LD-DKO Huh7 cells by infection with lentiviral vectors. Cells were infected with Con1-JFH1 or Jc1 at an MOI of 1, and intracellular HCV RNA levels were determined at 24 h post-infection by qRT-PCR. (F) Sera (100μl) from chimeric mice infected with HCV were inoculated into SR/LD-DKO Huh7 cells expressing either SR-B1, LDLR or VLDLR in 24 well plate. Intracellular HCV RNA levels were determined at 72 h post-infection. In all cases, asterisks indicate significant differences (*P<0.05; **P<0.01) versus the results for control cells.

Article Snippet: The primary human hepatocyte (PHH) was purchased from PhoenixBio.

Techniques: Expressing, Quantitative RT-PCR, Infection, Western Blot, Control

Typical expression images of immunochemistry staining in tissue microarrays. (A and B) Low expression of CD68 in MIA tissues. Original magnification, (A) ×100 and (B) ×200. (C and D) High expression of CD68 in LPA tissues. Original magnification, (C) ×100 and (D) ×200. (E) Negative expression of IL-6 in MIA tissues. Original magnification, ×200. (F) Positive expression of IL-6 in LPA tissues. Original magnification, ×200. (G) Negative expression of CSF-1 in MIA tissues. Original magnification, ×200. (H) Positive expression of CSF-1 in LPA tissues. Original magnification, ×200. (I) High E-cadherin expression in MIA tissues. Original magnification, ×100. (J) Low E-cadherin expression in LPA tissues. Original magnification, ×100. (K) High Snail expression in LPA tissues. Original magnification, ×100. (L) High MMP-2 expression in LPA tissues. Original magnification, ×100. CD, cluster of differentiation; MIA, minimally invasive adenocarcinoma; LPA, lepidic predominant adenocarcinoma; CSF, colony-stimulating factor; IL, interleukin; MMP, metalloproteinase.

Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: Typical expression images of immunochemistry staining in tissue microarrays. (A and B) Low expression of CD68 in MIA tissues. Original magnification, (A) ×100 and (B) ×200. (C and D) High expression of CD68 in LPA tissues. Original magnification, (C) ×100 and (D) ×200. (E) Negative expression of IL-6 in MIA tissues. Original magnification, ×200. (F) Positive expression of IL-6 in LPA tissues. Original magnification, ×200. (G) Negative expression of CSF-1 in MIA tissues. Original magnification, ×200. (H) Positive expression of CSF-1 in LPA tissues. Original magnification, ×200. (I) High E-cadherin expression in MIA tissues. Original magnification, ×100. (J) Low E-cadherin expression in LPA tissues. Original magnification, ×100. (K) High Snail expression in LPA tissues. Original magnification, ×100. (L) High MMP-2 expression in LPA tissues. Original magnification, ×100. CD, cluster of differentiation; MIA, minimally invasive adenocarcinoma; LPA, lepidic predominant adenocarcinoma; CSF, colony-stimulating factor; IL, interleukin; MMP, metalloproteinase.

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques: Expressing, Staining

(A) Kaplan-Meier analysis of overall survival for the combination of CD68, CSF-1 and IL-6. (B) Kaplan-Meier analysis of disease-free survival for the combination of CD68, CSF-1 and IL-6. CSF-1, colony-stimulating factor-1; CD, cluster of differentiation; IL, interleukin,

Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: (A) Kaplan-Meier analysis of overall survival for the combination of CD68, CSF-1 and IL-6. (B) Kaplan-Meier analysis of disease-free survival for the combination of CD68, CSF-1 and IL-6. CSF-1, colony-stimulating factor-1; CD, cluster of differentiation; IL, interleukin,

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques:

(A) OS in patients with AIS/MIA in the CD68+CSF-1+IL-6+ group compared with other groups, including the CD68-, CD68+CSF-1+IL-6-, CD68+CSF-1-IL-6+ and CD68+CSF-1-IL-6- groups. (B) DFS in patients with AIS/MIA in the CD68+ CSF-1+ IL-6+ group compared with other groups (C) Comparison of OS in AIS/MIA patients with CD68+CSF-1+IL-6+ and in LPA patients with CD68+CSF-1+IL-6+. (D) Comparison of DFS in AIS/MIA patients with CD68+CSF-1+IL-6+ and in LPA patients with CD68+CSF-1+IL-6+. *Others include the CD68+CSF-1+IL-6- group, the CD68+CSF-1-IL-6+ group, the CD68+CSF-1-IL-6- group and the CD68- group. AIS, adenocarcinoma in situ ; MIA, minimally invasive adenocarcinoma; CD, cluster of differentiation; CSF, colony-stimulating factor; IL, interleukin; OS, overall survival; DFS, disease-free survival.

Journal: Oncology Letters

Article Title: Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma

doi: 10.3892/ol.2016.5270

Figure Lengend Snippet: (A) OS in patients with AIS/MIA in the CD68+CSF-1+IL-6+ group compared with other groups, including the CD68-, CD68+CSF-1+IL-6-, CD68+CSF-1-IL-6+ and CD68+CSF-1-IL-6- groups. (B) DFS in patients with AIS/MIA in the CD68+ CSF-1+ IL-6+ group compared with other groups (C) Comparison of OS in AIS/MIA patients with CD68+CSF-1+IL-6+ and in LPA patients with CD68+CSF-1+IL-6+. (D) Comparison of DFS in AIS/MIA patients with CD68+CSF-1+IL-6+ and in LPA patients with CD68+CSF-1+IL-6+. *Others include the CD68+CSF-1+IL-6- group, the CD68+CSF-1-IL-6+ group, the CD68+CSF-1-IL-6- group and the CD68- group. AIS, adenocarcinoma in situ ; MIA, minimally invasive adenocarcinoma; CD, cluster of differentiation; CSF, colony-stimulating factor; IL, interleukin; OS, overall survival; DFS, disease-free survival.

Article Snippet: Immunohistochemistry was performed with mouse anti-human CD68 monoclonal antibody at 1:5 dilution (clone KP1; Abcam, Cambridge, UK), rabbit anti-human colony-stimulating factor (CSF)-1 polyclonal antibody at 1:100 dilution (BA0750; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-interleukin (IL)-6 at 1:100 dilution (BS0781R; BIOSS, Beijing, China), anti-matrix metalloproteinase (MMP)-2 at 1:400 dilution (ab37150; Abcam), anti-E-cadherin at 1:200 dilution (sc-7,870; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and anti-Snail at 1:25 dilution (AP2054a; Abgent, Inc., San Diego, CA, USA).

Techniques: In Situ